کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6392327 | 1330438 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid detection of Cronobacter sakazakii in dairy food by biofunctionalized magnetic nanoparticle based on nuclear magnetic resonance
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In order to ensure the safety of infant formula powder in China, a rapid and sensitive detection method for food-borne bacteria is urgently needed. We have developed a reliable immunoassay based on nuclear magnetic resonance for the specific detection of Enterobacter sakazakii in dairy samples with biofunctionalized magnetic nanoparticles. This method is able to detect Cronobacter sakazakii in milk powder and cheese samples at 1.1 to 11Â MPN using the most-probable-number (MPN) assay, within an incubation period of less than 2Â h. Longer incubation time (>4Â h) or higher pH (>7) will decrease the sensitivity of this method. This method does not fit for the detection of bacteria at higher concentrations (>1100Â MPN). This method has great potential to becomea useful tool for the rapid detection of bacterial contaminations in food, environmental and agricultural samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 34, Issue 2, December 2013, Pages 436-443
Journal: Food Control - Volume 34, Issue 2, December 2013, Pages 436-443
نویسندگان
Yu Zhao, Yuting Yao, Ming Xiao, Yan Chen, Charles C.C. Lee, Li Zhang, Kelvin Xi Zhang, Shiping Yang, Ming Gu,