A new multiplex real-time PCR developed method for Salmonella spp. and Listeria monocytogenes detection in food and environmental samples

► Simultaneous growth of Salmonella spp. and L. monocytogenes was achieved. ► A qPCR detection system for simultaneous detection of 5 cfu in 25 g was developed. ► Ninety-five food and environmental samples were analyzed.