Mixed culture enrichment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, and Yersinia enterocolitica

► Mixed culture enrichment desired for newer, multiplexed rapid methods. ► Target growth was 10e5 CFU/mL, a level greater than detection limit for most rapid methods. ► Compared are commercially available and custom-developed co-enrichment media. ► Except L. monocytogenes in pork, all pathogens grew beyond target concentration.