کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6394036 | 1330457 | 2013 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development of a PCR protocol to detect ochratoxin A producing moulds in food products Development of a PCR protocol to detect ochratoxin A producing moulds in food products](/preview/png/6394036.png)
Ochratoxin A (OTA) is a mycotoxin produced by several Penicillium and Aspergillus species growing in food commodities. To prevent OTA in foods it is necessary to have rapid and specific methods for early detection of producing moulds regardless of species and genera. In this work a PCR method to detect ochratoxigenic moulds has been developed. For this purpose, 75 mould strains belonging to species usually reported in food products were used. Their OTA production was checked by micellar electrokinetic capillary electrophoresis (MECE) and high-pressure liquid chromatography-mass spectrometry (HPLC-MS). A specific amplicon of 459Â bp was detected by using the designed PCR protocol only in the OTA producing strains. The detection limit of the developed PCR protocol was estimated for 25Â pg of mould DNA from pure cultures and from about 102-104Â cfu/g when it was evaluated directly on artificially inoculated food. Its functionality in naturally infected samples was also demonstrated. In conclusion, the developed PCR method could be used for detecting ochratoxigenic moulds in foods and consequently for monitoring these moulds in the HACCP programs.
Journal: Food Control - Volume 29, Issue 1, January 2013, Pages 270-278