Occurrence and characteristics of Vibrio vulnificus in retail marine shrimp in China

Vibrio vulnificus is a gram-negative bacterium that naturally occurs in estuarine waters and marine environment. Consumption of seafood contaminated with V. vulnificus or exposure to the contaminated water causes septicemia, gastroenteritis or wound infections in humans. This study aimed at identifying the prevalence of V. vulnificus in shrimps in Chinese seafood market. From June to September 2009, 239 samples encompassing six shrimp species were collected from ten cities in China. To quantify the prevalence and the levels of V. vulnificus in shrimps, the most probable number (MPN) method was used combining with the polymerase chain reaction (PCR) which was used to classify the isolates by detecting V. vulnificus hemolysin gene (vvhA). One hundred and forty out of 239 samples were positive for V. vulnificus, with the geometric mean level at 153.3 MPN/g. The prevalence and levels of V. vulnificus in shrimps varied in different geographic areas, with the values for the samples in the South cities (179.6 MPN/g) remarkably higher than that in the North cities (7.6 MPN/g). In addition, we found that 61.7% (79/128) of the V. vulnificus positive samples contained C-type (Clinical) strains and 8 contained E-type (Environmental) strains. The antimicrobial susceptibility of the isolates to 12 antibiotics was tested as well. Most of the 169 isolates remained susceptible to the majority of antimicrobials tested, whereas some strains tend to be less sensitive to amikacin, ampicillin, tetracycline and gentamicin. In conclusion, V. vulnificus is commonly found in retail marine shrimp in Chinese seafood market, and most of the shrimp samples contained C-type strains, which may pose a potential threat to human health.

► We determined the prevalence of Vibrio vulnificus in retail marine shrimps in China. ► We got the levels of V. vulnificus in retail marine shrimps from China. ► Most of the V. vulnificus positive samples contained C-type (Clinical) strains. ► Most of V. vulnificus strains remain susceptible to all 12 antimicrobials tested. ► A small number of strains tend to be less sensitive to four antimicrobials.