کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6394302 | 1330462 | 2012 | 6 صفحه PDF | دانلود رایگان |
Cyprinidae fish are widely distributed in Taiwan, and processed products made from them are favored by consumers. To identify the species used for these processed products, complete mitochondrial cytochrome b genes (cytb, 1141Â bp) of 8 Cyprinidae were established. Then, a rapid PCR-RFLP method was used to amplify a 426Â bp fragment of cytb using primer set Lnew1/Hnew1, and the fragment was co-digested with NlaIII and BstXI. The results show that using this PCR-RFLP technique for the species identification of the 8 Cyprinidae was successful. The method was further applied to investigate 16 commercial samples of processed Cyprinidae products from traditional markets. The results indicate that 9 of the samples were Carassius auratus auratus, Cyprinus carpio carpio, or Ctenopharyngodon idella. The other 6 samples did not belong to the Cyprinidae family according to PCR-RFLP. These samples were further analyzed with the forensically informative nucleotide sequencing (FINS) and BLAST methods. The results show that these 6 processed products were Oreochromis spp., a monophyletic fish group. The last commercial product could not be amplified to obtain a 426Â bp fragment, so its species remains unknown. This study shows that PCR-RFLP and FINS can be used to identify processed products fraudulently labeled as Cyprinidae.
⺠PCR-RFLP is a rapid and cheap method for species identification. ⺠FINS and BLAST can detect correct species of unknown products. ⺠We established the complete mitochondrial cytochrome b gene of 8 Cyprinidae fish.
Journal: Food Control - Volume 28, Issue 2, December 2012, Pages 240-245