کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6394612 1330470 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short communicationValidation of a Real-time PCR assay for fast and sensitive quantification of Brucella spp. in water buffalo milk
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Short communicationValidation of a Real-time PCR assay for fast and sensitive quantification of Brucella spp. in water buffalo milk
چکیده انگلیسی

Water buffalo milk and derived dairy products, including mozzarella cheese, represent a possible source of Brucella contamination for consumers. Brucella is a severe pathogen for human health even at low concentrations. It is therefore fundamental to develop an assay that is faster and more sensitive than the traditional bacterial culturing method for the detection of the pathogen in the food matrix. We designed a Real-time PCR assay able to detect as low as 1 CFU/ml of Brucella spp. in water (80% probability) and 3 CFU/ml of Brucella spp. in buffalo milk (50% probability) in less than 3 h without any enrichment step. The assay was validated by calculating specificity, sensitivity, detection limit, precision, PCR efficiency, DNA extraction efficiency and food matrix inhibition. When this method was employed to detect and quantify Brucella spp. in 109 buffalo milk samples, the assay demonstrated a higher sensitivity in comparison to bacteriological analysis (27 positive samples and 2 positive samples, respectively).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 22, Issue 8, August 2011, Pages 1466-1470
نویسندگان
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