کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6394966 1330629 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glycation of β-lactoglobulin under dynamic high pressure microfluidization treatment: Effects on IgE-binding capacity and conformation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Glycation of β-lactoglobulin under dynamic high pressure microfluidization treatment: Effects on IgE-binding capacity and conformation
چکیده انگلیسی


- The IgE-binding capacity of β-Lg after glycation and DHPM treatment was studied.
- Glycation combined with DHPM caused the unfolding and aggregation of β-Lg, follow by increased denaturation temperature.
- DHPM treatment decreased the IgE-binding capacity of β-Lg-galactose conjugates.

The effects of dynamic high-pressure microfluidization (DHPM) (80, 120, and 160 MPa) treatment and glycation with galactose on the IgE-binding capacity and conformation of β-lactoglobulin (β-Lg) were investigated. The binding capacity of immunoglobulin E (IgE) from patients' sera with cow's milk allergy on β-Lg glycated with galactose decreased after DHPM treatment. β-Lg treated after different DHPM methods and pressures yielded a significant discrepancy in IgE-binding capacity. When β-Lg was pretreated by DHPM, the IgE-binding capacity of β-Lg-galactose conjugates decreased with increasing pressure; however, the conjugates showed higher IgE-binding capacity at 120 MPa than that at 80 and 160 MPa when the β-Lg-galactose mixture was treated by DHPM. Results of thermal properties, intrinsic fluorescence spectroscopy, surface hydrophobicity, and circular dichroism (CD) spectra indicated the occurrence of protein unfolding, as well as the tertiary and secondary structural changes of β-Lg. The results suggested pretreatment by DHPM and glycation with galactose was a promising approach for eliminating the IgE-binding capacity of β-Lg.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 89, Part 1, November 2016, Pages 882-888
نویسندگان
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