|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|6397581||1628490||2013||11 صفحه PDF||سفارش دهید||دانلود رایگان|
A reversed phase HPLC-DAD coupled to APCI-MS method is reported for the determination of the degradation of Î²-carotene and characterization of the oxidation products in corn oil. Corn oil containing Î²-carotene was oxidized in the Rancimat at 110Â Â°C from 1 to 14Â h. A significant degradation of Î²-carotene was observed in corn oil during accelerated thermal oxidation. A total of eight different oxidized products of Î²-carotene were identified, which includes 8â²-apo-Î²-carotenal, 6â²-apo-Î²-carotenal, 5,6-epoxy-8â²-apo-Î²-carotenal, Î²-carotene-2,2â²-dione, 13-Z-5,6,5â²,6â²-diepoxy-Î²-carotene, all-E-5,8-epoxy-Î²-carotene, all-E-5,6-epoxy-Î²-carotene, and 15-Z-5,6-epoxy-Î²-carotene. Corn oil triacylglycerols (TAGs) oxidation products were also identified using isocratic HPLC-ESI-MS. It was found that Î²-carotene promoted oxidation of TAGs especially at longer exposure times. For the first time two new classes of oxidized TAGs have been identified in corn oil, which were epidioxy bis-hydroperoxides and hydroxy bis-hydroperoxides. Other oxidation products of TAGs were mono-epoxides, mono-hydroperoxides, and epoxy hydroperoxides. It was found that complementing the HPLC-DAD method for Î²-carotene with ESI-MS method for TAGs oxidation provides a comprehensive set of analytical tools to characterize carotenoids and triacylglycerols oxidation and degradation in edible oils.
Journal: Food Research International - Volume 50, Issue 2, March 2013, Pages 534-544