کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6451952 | 1416984 | 2017 | 6 صفحه PDF | دانلود رایگان |
- R. sphaeroides was engineered by heterologous expression of an alcohol dehydrogenase.
- Engineered R. sphaeroides could be activated by light to offer NAD(P)H.
- An alternative light-driven NAD(P)H regeneration system was successfully developed.
- This fully light-driven system can be used for cheap synthesis of chiral alcohol.
Herein, we reported that Rhodobacter sphaeroides (R. sphaeroides) can be engineered by heterologous expression of an alcohol dehydrogenase (adh) from Leifsonia sp. to build a light-driven cofactor regeneration system for synthesis of chiral alcohol. The model substrate, 3â²-chloroacetophenone, can be reduced by the engineered R. sphaeroides to (R)-1-(3-chlorophenyl) ethanol with an enantiomeric excess (e.e.) value of more than 99% in an n-hexane/aqueous biphasic media. This system, which is fully controlled by light, exhibited potential power to be an alternative cofactor regeneration platform for cheap synthesis of various chiral alcohols via the cloning other oxidoreductases with diverse characteristics.
Journal: Journal of Biotechnology - Volume 259, 10 October 2017, Pages 120-125