کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6452106 | 1416994 | 2017 | 6 صفحه PDF | دانلود رایگان |
- First instance of a C. difficile toxin locus being encoded by an associated bacteriophage.
- Putative mobility of toxins in C. difficile.
- Expression of genes associated with the toxins.
Pathogenic clostridia typically produce toxins as virulence factors which cause severe diseases in both humans and animals. Whereas many clostridia like e.g., Clostridium perfringens, Clostridium botulinum or Clostridium tetani were shown to contain toxin-encoding plasmids, only toxin genes located on the chromosome were detected in Clostridioides difficile so far. In this study, we determined, annotated, and analyzed the complete genome of the bacteriophage phiSemix9P1 using single-molecule real-time sequencing technology (SMRT). To our knowledge, this represents the first C. difficile-associated bacteriophage genome that carries a complete functional binary toxin locus in its genome.
Journal: Journal of Biotechnology - Volume 250, 20 May 2017, Pages 23-28