Short communicationProduction of uracil from methane by a newly isolated Methylomonas sp. SW1

- Methylomonas sp. SW1 produced uracil from methane as a sole carbon source under aerobic condition.
- The uracil production is depending on Cu2+ concentration and temperature.
- Uracil was produced with a titer of 2.1 mg/L in 84 h without genetic engineering under the optimized condition.

Methane is an abundant, inexpensive one-carbon feedstock and one of the most powerful greenhouse gases. Because it does not compete with food demand, it is considered a promising carbon feedstock for the production of valuable products using methanotrophic bacteria. Here, we isolated a novel methanotrophic bacterium, Methylomonas sp. SW1, from a sewage sample obtained from Wonju City Water Supply Drainage Center, Republic of Korea. The conditions for uracil production by Methylomonas sp. SW1, such as Cu2+ concentration and temperature were investigated and optimized. As a result, Methylomonas sp. SW1 produced uracil from methane as a sole carbon source with a titer of 2.1 mg/L in 84 h without genetic engineering under the optimized condition. The results in this study demonstrate the feasibility of using Methylomonas sp. SW1 for the production of uracil from methane. This is the first report of uracil production from gas feedstock by methanotrophic bacteria.