کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6462765 1422149 2017 18 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Independent validation of body fluid-specific CpG markers and construction of a robust multiplex assay
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Independent validation of body fluid-specific CpG markers and construction of a robust multiplex assay
چکیده انگلیسی
Potential forensic use of tissue-specific DNA methylation markers has recently been discussed for the identification of the biological source of a stain. In this study 13 promising markers were evaluated to identify suitable candidate markers for the development of a robust and reliable multiplex assay. The results of this study suggest that a combination of only four highly informative markers will be enough for clear body fluid identification. A multiplex assay was developed for the identification of menstrual blood, saliva, semen, and venous blood. This assay was successfully applied to the identification of these body fluids in mixtures and crime scene stains. The multiplex assay aids in the identification of not only single source body fluids but also of body fluid mixtures. The main advantage of using DNA methylation assays over alternative tests is that it can be applied at a later time point in the investigative process since testing is possible even after DNA analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 29, July 2017, Pages 261-268
نویسندگان
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