A systems chemical biology study of malate synthase and isocitrate lyase inhibition in Mycobacterium tuberculosis during active and NRP growth

- SCB related virtual screening and docking studies can provide mechanistic insight on inhibition.
- Unlike glyoxlate response, inhibitor impact on malate seems microenvironment dependent.
- For similar microenvironments, ICL versus MS inhibition is metabolically more consequential.
- Strong ICL inhibition causes metabolite levels in persistent Mtb toward levels in non-viable Mtb.
- SCB points to a complex interplay between network structure, kinetics, and therapeutic outcome.