کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6488191 1416361 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Maximize rhamnolipid production with low foaming and high yield
ترجمه فارسی عنوان
حداکثر تولید رحمانلیپید با فوم کم و عملکرد بالا
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Rhamnolipids are well-known microbial surfactants with many potential applications. Their production cost, however, remains high due to the severe foaming tendency in aerobic fermentation and the relatively low productivity and yield. In this study, we assessed the boundaries set by these constraints after optimization of basic parameters such as dissolved oxygen concentration (DO), pH and carbon sources. DO 10% and pH 5.5-5.7 were found optimal; cell growth and/or rhamnolipid production were slower at lower DO (5%) or pH (5.0) while foaming became hard to control at higher DO (30%) or pH (6.0 and 6.5). Although the Pseudomonas aeruginosa strain used was selected for its high rhamnolipid production from glycerol as substrate, soybean oil was still found to be a better substrate that increased specific rhamnolipid productivity to 25.8 mg/g cells-h from the glycerol-supported maximum of 8.9 mg/g cells-h. In addition, the foam volume was approximately halved by using soybean oil instead of glycerol as substrate. Analysis by liquid chromatography coupled with mass spectrometry revealed that rhamnolipid compositions from the two carbon sources were also very different, with primarily (82%) monorhamnolipids from soybean oil and more (64%) dirhamnolipids from glycerol. The optimized fermentation produced 42 g/l rhamnolipids at a yield of approximately 47% and a volumetric productivity of 220 mg/l-h. These values are among the highest reported.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 110, March 2018, Pages 79-86
نویسندگان
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