کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6490524 43371 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Salt stress induced lipid accumulation in heterotrophic culture cells of Chlorella protothecoides: Mechanisms based on the multi-level analysis of oxidative response, key enzyme activity and biochemical alteration
ترجمه فارسی عنوان
تجمع لیپید ناشی از استرس نمک در سلول های هتروتروفی فرهنگی پروتوتایکوئید کلرلا: مکانیسم های مبتنی بر تجزیه چند سطحی از پاسخ اکسیداتیو، فعالیت آنزیم کلیدی و تغییرات بیوشیمیایی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Salt stress as an effective stress factor that could improve the lipid content and lipid yield of glucose in the heterotrophic culture cells of Chlorella protothecoides was demonstrated in this study. The highest lipid content of 41.2% and lipid yield of 185.8 mg/g were obtained when C. protothecoides was stressed under 30 g/L NaCl condition at its late logarithmic growth phase. Moreover, the effects of salt and osmotic stress on lipid accumulation were comparatively analyzed, and it was found that the effects of NaCl and KCl stress had no significant differences at the same osmolarity level of 1150 mOsm/kg with lipid contents of 41.7 and 40.8% as well as lipid yields of 192.9 and 186.8 mg/g, respectively, whereas these results were obviously higher than those obtained under the iso-osmotic glycerol and sorbitol stresses. Furthermore, basing on the multi-level analysis of oxidative response, key enzyme activity and biochemical alteration, the superior performance of salt stress driving lipid over-synthesis was probably ascribed to the more ROS production as a result of additional ion effect besides the osmotic effect, subsequently mediating the alteration from carbohydrate storage to lipid accumulation in signal transduction process of C. protothecoides.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 228, 20 June 2016, Pages 18-27
نویسندگان
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