کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490601 | 43377 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis and optimization of triacylglycerol synthesis in novel oleaginous Rhodococcus and Streptomyces strains isolated from desert soil
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CDWTAGMSMTFATLCStreptomycesFAEECFUPHAPHBFatty Acid ethyl esters - اتیل استرهای اسید چربWax esters - استرهای مومTriacylglycerols - ترشیال گلیسیرینtriacylglycerol - تری آسیل گلیسرول Desert soil - خاک کویرFAME - فامlysogeny broth - لیزوزومی گوگردfatty acid methyl esters - متیل استرهای اسید چربmineral salt medium - مواد معدنی نمک متوسطnutrient broth - مواد مغذی براثOleaginous microorganisms - میکروارگانیسم های آلوده کنندهcolony forming units - واحدهای تشکیل دهنده کلنیcell dry weight - وزن خشک سلولیpoly(β-hydroxybutyrate) - پلی (β-هیدروکسی بوتیرات)polyhydroxyalkanoate - پلی هیدروکسی آلکینواتthin-layer chromatography - کروماتوگرافی نازک لایهGas chromatography - کروماتوگرافی گازیTotal fatty acids - کل اسیدهای چرب
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
As oleaginous microorganisms represent an upcoming novel feedstock for the biotechnological production of lipids or lipid-derived biofuels, we searched for novel, lipid-producing strains in desert soil. This was encouraged by the hypothesis that neutral lipids represent an ideal storage compound, especially under arid conditions, as several animals are known to outlast long periods in absence of drinking water by metabolizing their body fat. Ten lipid-accumulating bacterial strains, affiliated to the genera Bacillus, Cupriavidus, Nocardia, Rhodococcus and Streptomyces, were isolated from arid desert soil due to their ability to synthesize poly(β-hydroxybutyrate), triacylglycerols or wax esters. Particularly two Streptomyces sp. strains and one Rhodococcus sp. strain accumulate significant amounts of TAG under storage conditions under optimized cultivation conditions. Rhodococcus sp. A27 and Streptomyces sp. G49 synthesized approx. 30% (w/w) fatty acids from fructose or cellobiose, respectively, while Streptomyces isolate G25 reached a cellular fatty acid content of nearly 50% (w/w) when cultivated with cellobiose. The stored triacylglycerols were composed of 30-40% branched fatty acids, such as anteiso-pentadecanoic or iso-hexadecanoic acid. To date, this represents by far the highest lipid content described for streptomycetes. A biotechnological production of such lipids using (hemi)cellulose-derived raw material could be used to obtain sustainable biodiesel with a high proportion of branched-chain fatty acids to improve its cold-flow properties and oxidative stability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 225, 10 May 2016, Pages 48-56
Journal: Journal of Biotechnology - Volume 225, 10 May 2016, Pages 48-56
نویسندگان
Annika Röttig, Philippa Hauschild, Mohamed H. Madkour, Ahmed M. Al-Ansari, Naief H. Almakishah, Alexander Steinbüchel,