کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490965 | 43388 | 2015 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rescuing chemotaxis of the anticancer agent Salmonella enterica serovar Typhimurium VNP20009
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rescuing chemotaxis of the anticancer agent Salmonella enterica serovar Typhimurium VNP20009 Rescuing chemotaxis of the anticancer agent Salmonella enterica serovar Typhimurium VNP20009](/preview/png/6490965.png)
چکیده انگلیسی
The role of chemotaxis and motility in Salmonella enterica serovar Typhimurium tumor colonization remains unclear. We determined through swim plate assays that the well-established anticancer agent S. Typhimurium VNP20009 is deficient in chemotaxis, and that this phenotype is suppressible. Through genome sequencing, we revealed that VNP20009 and four selected suppressor mutants had a single nucleotide polymorphism (SNP) in cheY causing a mutation in the conserved proline residue at position 110. CheY is the response regulator that interacts with the flagellar motor-switch complex and modulates rotational bias. The four suppressor mutants additionally carried non-synonymous SNPs in fliM encoding a flagellar switch protein. The CheY-P110S mutation in VNP20009 likely rendered the protein unable to interact with FliM, a phenotype that could be suppressed by mutations in FliM. We replaced the mutated cheY in VNP20009 with the wild-type copy and chemotaxis was partially restored. The swim ring of the rescued strain, VNP20009 cheY+, was 46% the size of the parental strain 14028 swim ring. When tested in capillary assays, VNP20009 cheY+ was 69% efficient in chemotaxis towards the attractant aspartate as compared to 14028. Potential reasons for the lack of complete restoration and implications for bacterial tumor colonization will be discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 211, 10 October 2015, Pages 117-120
Journal: Journal of Biotechnology - Volume 211, 10 October 2015, Pages 117-120
نویسندگان
Katherine M. Broadway, Elizabeth A.P. Denson, Roderick V. Jensen, Birgit E. Scharf,