A new application of aptamer: One-step purification and immobilization of enzyme from cell lysates for biocatalysis

- An improved and simple protocol for DNA aptamer selection was developed.
- One-step purification and immobilization of PGUS-E from cell lysates was achieved based aptamer modified magnetic beads.
- A purification procedure by aptamer-based magnetic beads was established by simply manipulating pH and ionic concentration.
- The immobilized PGUS-E via aptamer showed high operational stability and support regeneration stability.