کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491200 | 43400 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro production and antifungal activity of peptide ABP-dHC-cecropin A
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کلمات کلیدی
SUMOElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزsmall ubiquitin-related modifier - اصلاح کننده کوچک کوچک ubiquitinEscherichia coli expression - بیان اشرشیا کولیEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکAntifungal activity - فعالیت ضد قارچیMALDI–TOF MS - مالدی توف MSSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیScanning electron microscopy - میکروسکوپ الکترونی روبشیAntibacterial peptide - پپتید ضد باکتری
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The antimicrobial peptide ABP-dHC-cecropin A is a small cationic peptide with potent activity against a wide range of bacterial species. Evidence of antifungal activity has also been suggested; however, testing of this peptide has been limited due to the low expression of cecropin proteins in Escherichia coli. To improve expression of this peptide in E. coli, ABP-dHC-cecropin A was cloned into a pSUMO vector and transformed into E. coli, resulting in the production of a pSUMO-ABP-dHC-cecropin A fusion protein. The soluble form of this protein was then purified by Ni-IDA chromatography, yielding a total of 496-mg protein per liter of fermentation culture. The SUMO-ABP-dHC-cecropin A fusion protein was then cleaved using a SUMO protease and re-purified by Ni-IDA chromatography, yielding a total of 158-mg recombinant ABP-dHC-cecropin A per liter of fermentation culture at a purity of â¥94%, the highest yield reported to date. Antifungal activity assays performed using this purified recombinant peptide revealed strong antifungal activity against both Candida albicans and Neurospora crassa, as well as Rhizopus, Fusarium, Alternaria, and Mucor species. Combined with previous analyses demonstrating strong antibacterial activity against a number of important bacterial pathogens, these results confirm the use of ABP-dHC-cecropin A as a broad-spectrum antimicrobial peptide, with significant therapeutic potential.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 199, 10 April 2015, Pages 47-54
Journal: Journal of Biotechnology - Volume 199, 10 April 2015, Pages 47-54
نویسندگان
Jiaxin Zhang, Ali Movahedi, Junjie Xu, Mengyang Wang, Xiaolong Wu, Chen Xu, Tongming Yin, Qiang Zhuge,