کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491528 | 43412 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabolic switching of central carbon metabolism in response to nitrate: Application to autofermentative hydrogen production in cyanobacteria
ترجمه فارسی عنوان
تعویض متابولیک متابولیسم مرکزی کربن در واکنش به نیترات: کاربرد تولید هیدروژن زا در سیانوباکتری
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Nitrate removal from culture media is widely used to enhance autofermentative hydrogen production in cyanobacteria during dark anaerobiosis. Here we have performed a systematic inventory of carbon and nitrogen metabolites, redox pools, and excreted product fluxes which show that addition of nitrate to cultures of Synechococcus sp. PCC 7002 has no influence on glycogen catabolic rate, but shifts the distribution of excreted products from predominantly lactate and H2 to predominantly CO2 and nitrite, while increasing the total consumption of intracellular reducing equivalents (mainly glycogen) by 3-fold. Together with LC-MS derived metabolite pool sizes these data show that glycogen catabolism is redirected from the upper-glycolytic (EMP) pathway to the oxidative pentose phosphate (OPP) pathway upon nitrate addition. This metabolic switch in carbon catabolism is shown to temporally correlate with the pyridine nucleotide redox-poise (NAD(P)H/NAD(P)+) and demonstrates the reductant availability controls H2 evolution in cyanobacteria.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volumes 182â183, 20 Julyâ10 August 2014, Pages 83-91
Journal: Journal of Biotechnology - Volumes 182â183, 20 Julyâ10 August 2014, Pages 83-91
نویسندگان
Kelsey McNeely, G. Kenchappa Kumaraswamy, Tiago Guerra, Nicholas Bennette, Gennady Ananyev, G. Charles Dismukes,