Development of a non-viral gene delivery vector based on the dynein light chain Rp3 and the TAT peptide

- Modular recombinant proteins are promising tools for non-viral gene delivery.
- pDNA:T-Rp3 complexes interact in vitro with the dynein intermediate chain.
- T-Rp3 efficiently delivers pDNA to HeLa cells with low cytotoxicity.
- Transfection is highly dependent on microtubule polarization.