کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6491621 43434 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Investigating the feasibility of scale up and automation of human induced pluripotent stem cells cultured in aggregates in feeder free conditions
ترجمه فارسی عنوان
بررسی امکان سنجی افزایش و خودکار سازی سلول های بنیادی انعقادی ناشی از انسداد انسانی در شرایط آزاد فیدر
کلمات کلیدی
سلول های بنیادی پلوروپتوژن منجر شده، فیدر رایگان اتوماسیون، افزایش مقیاس، ساخت،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The transfer of a laboratory process into a manufacturing facility is one of the most critical steps required for the large scale production of cell-based therapy products. This study describes the first published protocol for scalable automated expansion of human induced pluripotent stem cell lines growing in aggregates in feeder-free and chemically defined medium. Cells were successfully transferred between different sites representative of research and manufacturing settings; and passaged manually and using the CompacT SelecT automation platform. Modified protocols were developed for the automated system and the management of cells aggregates (clumps) was identified as the critical step. Cellular morphology, pluripotency gene expression and differentiation into the three germ layers have been used compare the outcomes of manual and automated processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 173, 10 March 2014, Pages 53-58
نویسندگان
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