کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491773 | 43447 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cultivation of shear stress sensitive microorganisms in disposable bag reactor systems
ترجمه فارسی عنوان
کشت میکروارگانیسم های حساس به تنش برشی در سیستم های راکتور کیسه ای یکبار مصرف
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Technical scale (â¥5 l) cultivations of shear stress sensitive microorganisms are often difficult to perform, as common bioreactors are usually designed to maximize the oxygen input into the culture medium. This is achieved by mechanical stirrers, causing high shear stress. Examples for shear stress sensitive microorganisms, for which no specific cultivation systems exist, are many anaerobic bacteria and fungi, such as basidiomycetes. In this work a disposable bag bioreactor developed for cultivation of mammalian cells was investigated to evaluate its potential to cultivate shear stress sensitive anaerobic Eubacterium ramulus and shear stress sensitive basidiomycetes Flammulina velutipes and Pleurotus sapidus. All cultivations were compared with conventional stainless steel stirred tank reactors (STR) cultivations. Good growth of all investigated microorganisms cultivated in the bag reactor was found. E. ramulus showed growth rates of μ = 0.56 hâ1 (bag) and μ = 0.53 hâ1 (STR). Differences concerning morphology, enzymatic activities and growth in fungal cultivations were observed. In the bag reactor growth in form of small, independent pellets was observed while STR cultivations showed intense aggregation. F. velutipes reached higher biomass concentrations (21.2 g lâ1 DCW vs. 16.8 g lâ1 DCW) and up to 2-fold higher peptidolytic activities in comparison to cell cultivation in stirred tank reactors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 167, Issue 4, 20 September 2013, Pages 370-376
Journal: Journal of Biotechnology - Volume 167, Issue 4, 20 September 2013, Pages 370-376
نویسندگان
Patrick Jonczyk, Meike Takenberg, Steffen Hartwig, Sascha Beutel, Ralf G. Berger, Thomas Scheper,