کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6491778 | 43447 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Efficient production of (R)-(â)-mandelic acid in biphasic system by immobilized recombinant E. coli
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Efficient production of (R)-(â)-mandelic acid in biphasic system by immobilized recombinant E. coli Efficient production of (R)-(â)-mandelic acid in biphasic system by immobilized recombinant E. coli](/preview/png/6491778.png)
چکیده انگلیسی
The recombinant Escherichia coli M15/BCJ2315 which harbored a mandelonitrilase from Burkholderia cenocepacia J2315 was immobilized via catecholic chitosan and functionalized with magnetism by iron oxide nanoparticles. The immobilized cells showed high activity recovery, enhanced stability and good operability in the enantioselective hydrolysis of mandelonitrile to (R)-(â)-mandelic acid. Furthermore, the immobilized cells were reused up to 15 cycles without any activity loss in completely hydrolyzing mandelonitrile (100 mM) within 1 h in aqueous solution. The ethyl acetate-water biphasic system was built and optimized. Under the optimal conditions, as high as 1 M mandelonitrile could be hydrolyzed within 4 h with a final yield and ee value of 99% and 95%, respectively. Moreover, the successive hydrolysis of mandelonitrile was performed by repeated use of the immobilized cells for 6 batches, giving a final productivity (g Lâ1 hâ1) and relative production (g gâ1) of 40.9 and 38.9, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 167, Issue 4, 20 September 2013, Pages 433-440
Journal: Journal of Biotechnology - Volume 167, Issue 4, 20 September 2013, Pages 433-440
نویسندگان
Kefeng Ni, Hualei Wang, Li Zhao, Minjie Zhang, Siyuan Zhang, Yuhong Ren, Dongzhi Wei,