Probing the metabolism of an inducible mammalian expression system using extracellular isotopomer analysis

► Metabolic characterization of an inducible mammalian expression system. ► CHO cells grown in parallel semi-continuous cultures with various labeled substrates. ► Metabolic flux analysis using 13C-tracers and extracellular isotopomer analysis. ► Induced cells exhibited a more efficient nutrient utilization and greater TCA fluxes.