کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6491869 43458 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of coxsackievirus and adenovirus receptor (CAR)-Fc fusion protein in Pichia pastoris and characterization of its anti-coxsackievirus activity
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Expression of coxsackievirus and adenovirus receptor (CAR)-Fc fusion protein in Pichia pastoris and characterization of its anti-coxsackievirus activity
چکیده انگلیسی
► Coding sequence of CAR-Fc fusion protein was optimized for expression in Pichia pastoris. ► CAR-Fc fusion protein has been successfully expressed, purified and identified in Pichia pastoris expression system. ► Purified CAR-Fc fusion protein was confirmed to have the binding activity with CVB3 directly. ► CAR-Fc fusion protein was confirmed to have bioactivity in blocking CVB3 virus infection in vitro and in vivo, with reduced mortality, myocardial edema, viral loads and inflammation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 164, Issue 4, 15 April 2013, Pages 461-468
نویسندگان
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