ELP-z and ELP-zz capturing scaffolds for the purification of immunoglobulins by affinity precipitation

► A new set of thermo-responsive ELP-z/zz domain fusions was constructed as the capturing scaffolds for the direct purification and recovery of antibodies. ► The ELP-zz scaffolds was used in four repeated binding and elution cycles with no observable decrease in the purification performance. ► The same binding and elution cycle was successfully implemented for the purification of monoclonal antibodies from hybridoma culture supernatant with close to 100% recovery.