Engineered biosealant strains producing inorganic and organic biopolymers

► Microbial urease plays a critical role in calcite precipitation in the environment. ► Sporosarcina pasteurii urease genes were cloned into Pseudomonas strains. ► Analyses confirmed recombinants produced urease, comparable to S. pasteurii. ► The recombinant Pseudomonas produced organic polymers and precipitated calcite. ► SEM evidenced organo-mineral biopolymers formation on the surface of recombinants.