کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6492042 | 43455 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alterations of glucose metabolism in Escherichia coli mutants defective in respiratory-chain enzymes
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کلمات کلیدی
TCAICDHNADH dehydrogenasePDHcNDHPMFTLCtricarboxylic acid - اسید تری کربوکسیلیکEscherichia coli - اشریشیا کُلیIsocitrate dehydrogenase - ایزوسیترات دهیدروژنازRespiratory chain - زنجیر تنفسیCitrate synthase - سیترات سیتواستاتCentral metabolism - متابولیسم مرکزی2-oxoglutarate dehydrogenase complex - مجتمع 2-اگزوگلوترات دهیدروژنازProton-motive force - نیروی حرکتی پروتونpyruvate dehydrogenase complex - پیرووات دهیدروژناز پیچیدهthin-layer chromatography - کروماتوگرافی نازک لایه
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Alterations of glucose metabolism in Escherichia coli mutants defective in respiratory-chain enzymes Alterations of glucose metabolism in Escherichia coli mutants defective in respiratory-chain enzymes](/preview/png/6492042.png)
چکیده انگلیسی
The effects of reduced efficiency of proton-motive force (pmf) generation on glucose metabolism were investigated in Escherichia coli respiratory-chain mutants. The respiratory chain of E. coli consists of two NADH dehydrogenases and three terminal oxidases, all with different abilities to generate a pmf. The genes for isozymes with the highest pmf-generating capacity (NADH dehydrogenase-1 and cytochrome bo3 oxidase) were knocked out singly or in combination, using a wild-type strain as the parent. Analyses of glucose metabolism by jar-fermentation revealed that the glucose consumption rate per cell increased with decreasing efficiency of pmf generation, as determined from the growth parameters of the mutants. The highest rate of glucose metabolism was observed in the double mutant, and the lowest was observed in the wild-type strain. The respiration rates of the single-knockout mutants were comparable to that of the wild-type strain, and that of the double mutant was higher, apparently as a result of the upregulation of the remaining respiratory chain enzymes. All of the strains excreted 2-oxoglutaric acid as a product of glucose metabolism. Additionally, all of the mutants excreted pyruvic acid and/or acetic acid. Interestingly, the double mutant excreted l-glutamic acid. Alterations of the fermentation profiles provide clues regarding the metabolic regulation in each mutant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 158, Issue 4, 30 April 2012, Pages 215-223
Journal: Journal of Biotechnology - Volume 158, Issue 4, 30 April 2012, Pages 215-223
نویسندگان
Chie Kihira, Yukari Hayashi, Naoki Azuma, Sakiko Noda, Soya Maeda, Satoru Fukiya, Masaru Wada, Kazunobu Matsushita, Atsushi Yokota,