The effects of ROS-mediating oxygen tension on human CD34+CD38− cells induced into mature dendritic cells

► The effect of ROS-mediating pO2 on HSPCs gene expression was studied by microarrays. ► Cells differentiation genes were down-regulated in hypoxia or reducing ROS group. ► Three genes related to dendritic cells maturation were confirmed by real-time PCR. ► Mature potential of DCs was suppressed by hypoxia-reducing ROS in further study. ► Hypoxia-reducing ROS regulated the genes driving HSPCs differentiation.