کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6493289 | 1418050 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tobramycin mediated silver nanospheres/graphene oxide composite for synergistic therapy of bacterial infection
ترجمه فارسی عنوان
توبرامایسین، نانوسفرهای نقره / کامپوزیت اکسید گرافین را برای درمان سینرژیک عفونت باکتریایی
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کلمات کلیدی
اکسید گرافن، ترومایسین، نانوذرات نقره، تعیین مشخصات، فعالیت ضد باکتری،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Graphene-based materials have attracted a significant attention in constructing hybrid systems for drug delivery with enhanced antimicrobial activities. In our work, we demonstrated the formation of silver nanoparticles (AgNPs) on graphene oxide (GO) using tobramycin (TOB), an aminoglycoside antibiotic, as reducing and decorating agent. The TOB decorated GO AgNPs (TOB-GO-Ag) composite was used as an antibacterial agent against multi-drug resistant Gram-negative E-coli (BL21 DE3). The reversal of surface potential from â30â¯mV (GO) to +20â¯mV confirms the successful reduction of GO by TOB. Atomic force microscopy (AFM) and high-resolution transmission electron microscopic (HRTEM) analyses confirmed the formation of uniformly distributed AgNPs on the reduced GO with an approximate particle size of 5â¯nm. The as-synthesized nanocomposite displayed significant antibacterial activity as compared to pure AgNPs and TOB. The positively charged TOB-GO-Ag interacts with the negatively charged E. coli membrane and inhibit bacterial growth by the antibacterial actions of the released silver, GO and tobramycin from the TOB-GO-Ag composite. The significant loss of bacterial membrane potential from â52â¯Â±â¯2â¯mV (control) to â2â¯Â±â¯1â¯mV (treated) indicates a severe cell wall damage caused by TOB-GO-Ag composite. Furthermore, fluorescence study also demonstrated a severe membrane disruption in bacterial cells treated with TOB-GO-Ag composite as compared to pure AgNPs and GO. In conclusion, the development of such hybrid systems would help in enhancing the efficacy of available drugs and eradicating the emerging bacterial resistance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 183, June 2018, Pages 342-348
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 183, June 2018, Pages 342-348
نویسندگان
Sadeeq Ullah, Aftab Ahmad, Fazli Subhan, Aminullah Jan, Muslim Raza, Arif Ullah Khan, Aziz-Ur Rahman, Usman Ali Khan, Muhammad Tariq, Qipeng Yuan,