کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494693 | 44817 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabolic engineering of Arabidopsis for butanetriol production using bacterial genes
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کلمات کلیدی
ADTArabinoseMCsBFDADGGC–MS - GC-MSPCA - PCAArabidopsis - آرابیدوپسیس یا رشادیPrincipal component analysis - تحلیل مولفههای اصلی یا PCADehydrogenase - دهیدروژنازmultiple cloning site - سایت کلونینگ چندگانهgas chromatography coupled to mass spectrometry - کروماتوگرافی گاز همراه با طیف سنجی جرمیXylose - گزیلوز
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Metabolic engineering of Arabidopsis for butanetriol production using bacterial genes Metabolic engineering of Arabidopsis for butanetriol production using bacterial genes](/preview/png/6494693.png)
چکیده انگلیسی
1,2,4-butanetriol (butanetriol) is a useful precursor for the synthesis of the energetic material butanetriol trinitrate and several pharmaceutical compounds. Bacterial synthesis of butanetriol from xylose or arabinose takes place in a pathway that requires four enzymes. To produce butanetriol in plants by expressing bacterial enzymes, we cloned native bacterial or codon optimized synthetic genes under different promoters into a binary vector and stably transformed Arabidopsis plants. Transgenic lines expressing introduced genes were analyzed for the production of butanetriol using gas chromatography coupled to mass spectrometry (GC-MS). Soil-grown transgenic plants expressing these genes produced up to 20 µg/g of butanetriol. To test if an exogenous supply of pentose sugar precursors would enhance the butanetriol level, transgenic plants were grown in a medium supplemented with either xylose or arabinose and the amount of butanetriol was quantified. Plants expressing synthetic genes in the arabinose pathway showed up to a forty-fold increase in butanetriol levels after arabinose was added to the medium. Transgenic plants expressing either bacterial or synthetic xylose pathways, or the arabinose pathway showed toxicity symptoms when xylose or arabinose was added to the medium, suggesting that a by-product in the pathway or butanetriol affected plant growth. Furthermore, the metabolite profile of plants expressing arabinose and xylose pathways was altered. Our results demonstrate that bacterial pathways that produce butanetriol can be engineered into plants to produce this chemical. This proof-of-concept study for phytoproduction of butanetriol paves the way to further manipulate metabolic pathways in plants to enhance the level of butanetriol production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 20, November 2013, Pages 109-120
Journal: Metabolic Engineering - Volume 20, November 2013, Pages 109-120
نویسندگان
Salah E. Abdel-Ghany, Irene Day, Adam L. Heuberger, Corey D. Broeckling, Anireddy S.N. Reddy,