Yihx-encoded haloacid dehalogenase-like phosphatase HAD4 from Escherichia coli is a specific α-d-glucose 1-phosphate hydrolase useful for substrate-selective sugar phosphate transformations

- Functional expression of Escherichia coli haloacid dehalogenase-like phosphatase 4 (HAD4).
- Characterization of HAD4 fusion to the cationic module Zbasic2.
- Single-step capture and polishing purification of Zbasic2_HAD4.
- Selective conversion of α-d-glucose 1-phosphate in mixture with glucose 6-phosphate.
- Oriented immobilization with high effectiveness of Zbasic2_HAD4 on porous support.