Facile immobilization of enzyme by entrapment using a plasma-deposited organosilicon thin film

- An original strategy of enzyme immobilization by entrapment in plasma-polymerized thin films is proposed.
- RPECVD is used to obtain the ppTMDSO thin films overcoating the adsorbed β-galactosidase.
- The role of both enzyme solvent and adsorption support is investigated.
- The enzyme leaching from the support and its resulting activity are analyzed as function of various coating thicknesses.
- β-galactosidase activity is characterized by studying its stability, the diffusional limitations and the kinetic parameters.