Biocatalytic production of 5-hydroxy-2-adamantanone by P450cam coupled with NADH regeneration

- We constructed Escherichia coli cells that expressed P450cam monooxygenase and its redox partners.
- We also constructed cells that co-expressed this P450cam multicomponent system with a glucose dehydrogenase (Gdh).
- Wet cells were able to effectively oxidize 2-adamantanone without co-expression of Gdh in the presence of glucose.
- In contrast, freeze-dried cells required co-expression of Gdh for the oxidation activity.
- These whole-cell catalysts attained gram-per-liter-scale production of 5-hydroxy-2-adamantanone.