کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6553255 1422144 2018 35 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Predicting the origin of stains from next generation sequencing mRNA data
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Predicting the origin of stains from next generation sequencing mRNA data
چکیده انگلیسی
We used our previously published NGS mRNA approach for body fluid identification to analyse 183 body fluids/tissues, including mock casework samples. The resulting data set was used to build a probabilistic model that predicts the origin of a stain. Our approach uses partial least squares followed by linear discriminant analysis to classify samples into six commonly occurring forensic body fluids. The model differs from the ones previously suggested in that it incorporates quantitative information (NGS read counts) rather than just presence/absence of markers. The suggested approach also allows for visualisation of important markers and their correlation with the different body fluids. We compared our model to previously published methods to show that the inclusion of read count information improves the prediction. Finally, we applied the model to mixed body fluid samples to test its ability to identify the individual components in a mixture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 34, May 2018, Pages 37-48
نویسندگان
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