کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6553875 160890 2015 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced low-template DNA analysis conditions and investigation of allele dropout patterns
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Enhanced low-template DNA analysis conditions and investigation of allele dropout patterns
چکیده انگلیسی
We find allele dropout probability differences between several STR markers. Markers labelled with the fluorescent dyes CXR-ET (red in electropherogram) and TMR-ET (shown as black) generally have higher dropout risks compared with those labelled with JOE (green) and fluorescein (blue). Overall, the marker D10S1248 has the lowest allele dropout probability and D8S1179 the highest. The marker effect is mainly pronounced for 30-32 PCR cycles. Such effects would not be expected if the amplification efficiencies were identical for all markers. Understanding allele dropout risks and the variability in peak heights and balances is important for correct interpretation of forensic DNA profiles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International: Genetics - Volume 14, January 2015, Pages 61-75
نویسندگان
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