کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6555481 | 1422483 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous co-detection of wild-type and vaccine strain measles virus using the BD MAX system
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موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی قانونی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Despite the reported elimination of measles virus in Australia, importation of cases from endemic countries continues to lead to secondary local transmission and outbreaks. Rapid laboratory confirmation of measles is paramount for individual patient management and outbreak responses. Further, it is important to rapidly distinguish infection from wild-type virus or vaccine strains to guide public health responses. We developed a high throughput, TaqMan-based multiplex reverse-transcription-polymerase chain reaction (PCR) assay using the BD MAX platform (Becton Dickinson) that simultaneously detects measles virus and differentiates between wild-type and vaccine strains without the need for sequencing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pathology - Volume 50, Issue 4, June 2018, Pages 450-454
Journal: Pathology - Volume 50, Issue 4, June 2018, Pages 450-454
نویسندگان
Kiran Thapa, Justin A. Ellem, Kerri Basile, Ian Carter, Tom Olma, Sharon C.-A. Chen, Dominic E. Dwyer, Jen Kok,