کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6609288 | 459545 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Geobacter sulfurreducens adapts to low electrode potential for extracellular electron transfer
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
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چکیده انگلیسی
Microbial extracellular electron transfer (EET) occurring in natural and engineering processes is attracting increasing interests. While a meaningful question for bioenergetics, microbial physiology and microbial electrochemical systems; less is known about the lower limit of electron acceptor reduction potential for EET. It is also unclear how microbes adapt to weak electron acceptors. This study evaluated Geobacter sulfurreducens biofilms grown with an electrode poised at â0.25 V vs. SHE. This potential was found to be sufficient for microbial metabolism and proliferation. The turnover cyclic voltammetries found that these biofilms had a half-saturation potential of â0.242 ± 0.004 V, in contrast to â0.151 ± 0.003 V for that of the biofilms grown under 0.2 V. For the biofilms grown under 0.2 V, differential pulse voltammetry showed that the metabolic current was mediated by interfacial cofactors with mid-point potential around â0.16 V performing single-electron electron transfer (ET). The major electron conduits for the biofilms respiring under â0.25 V had mid-point potentials of â0.22 V or â0.26 V, which appeared to perform two-electron ET. Under the non-turnover condition, both biofilms showed similar patterns in voltammograms and the low-potential conduits largely disappeared for the biofilms grown under â0.25 V. Transcriptome analysis identified 17 cytochrome-c genes significantly up-regulated for the biofilms grown under â0.25 V, together with many other genes linked to the ET system. It was also noted that, lowering the poised potential from â0.25 V to â0.28 V (the fuel standard oxidation potential) did not fully inhibit microbial respiration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 191, 10 February 2016, Pages 743-749
Journal: Electrochimica Acta - Volume 191, 10 February 2016, Pages 743-749
نویسندگان
Luo Peng, Xiao-Ting Zhang, Jie Yin, Shuo-Yuan Xu, Yong Zhang, De-Ti Xie, Zhen-Lun Li,