کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6615010 459616 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A high-sensitivity immunosensor for detection of tumor marker based on functionalized mesoporous silica nanoparticles
ترجمه فارسی عنوان
یک حساس ایمنی حساس به تشخیص تومور مارکر بر اساس نانوذرات سیلیکا مزپوروز
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی
The development of materials for the ultra sensitive detection of biological species has received great attention. Here, a new electrochemical immunosensor for sensitive detection of tumor marker was reported. Polydopamine (PDA) coating was used to modify indium tin oxide (ITO) electrode for functionalized mesoporous silica nanoparticles immobilization. The carcinoembryonic antigen (CEA) was used as a model analyte. The capture anti-CEA was immobilized on functionalized mesoporous silica nanoparticles. The horseradish peroxidase (HRP)-labeled signal anti-CEA antibodies were used to conjugated with gold nanoparticles. After the sandwich immunoreaction, under optimized conditions, the proposed immunosensor showed a high sensitivity and a linear range of 0.01-40.0 ng/mL with a detection limit of 1.7 pg/mL. The assay results of clinical serum samples obtained by the immunosensor we proposed were in acceptable agreement with the reference values. The designed immunoassay system with ultrahigh sensitivity and efficient and economical, might have potentially broad applications in protein diagnostics and bioassay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 112, 1 December 2013, Pages 473-479
نویسندگان
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