کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6618290 | 459654 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced direct electron transfer between laccase and hierarchical carbon microfibers/carbon nanotubes composite electrodes. Comparison of three enzyme immobilization methods
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Three immobilization protocols were investigated with respect to direct electron transfer between hierarchical carbon microfibers/carbon nanotubes composite material on graphite rod electrodes and Trametes hirsuta laccase. Immobilization was done by covalent binding of laccase to aminophenyl-modified electrodes via amide-bond formation with carboxylic acid residues or imino-bond formation with aldehyde groups introduced by oxidation of sugar residues of the enzyme's glycosylation shell. Moreover, immobilization was achieved by adsorbing laccase to electrodes hydrophilized with pyrene-hexanoic acid. High current densities for biocatalytic oxygen reduction were obtained for all immobilization strategies. The formation of the imino bonds let to the binding of laccase in close to 100% direct electron transfer configuration and consequently to the highest oxygen reduction currents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 82, 1 November 2012, Pages 218-223
Journal: Electrochimica Acta - Volume 82, 1 November 2012, Pages 218-223
نویسندگان
Cristina Gutiérrez-Sánchez, Wenzhi Jia, Yvonne Beyl, Marcos Pita, Wolfgang Schuhmann, Antonio L. De Lacey, Leonard Stoica,