کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6828871 | 548569 | 2008 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for altered post-receptor modulation of the serotonin 2a receptor in schizophrenia
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کلمات کلیدی
DOIHTR2ANSBnon-specific binding - اتصال غیر اختصاصیSchizophrenia - اسکیزوفرنی یا شیزوفرنیETE - تابستانPMI - شرکتهای کوچک و متوسطPostmortem interval - فاصله زمانی PostmortemCortex - قشرtotal binding - مجموع اتصالduration of illness - مدت زمان بیماریBrodmann's area - منطقه برادمنPostmortem - پس از مرگReceptor - گیرنده serotonin2a receptor - گیرنده سروتونین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
We have shown a decrease in cortical serotonin2A receptors using tissue sections, but not with washed membranes, from the same cohort of subjects. These discrepant findings led us to determine if we could obtain similar results using samples from the same tissue block. Our studies used single-point saturation analyses to estimate the total number of [3H]ketanserin binding sites in tissue sections, crude homogenate, membrane-enriched and cytosol-enriched tissue samples from Brodmann's area 9. There were significant decreases in the levels of [3H]ketanserin binding using tissue sections (mean ± SD: 38 ± 16 vs. 56 ± 16 fmol/mg ETE; p = 0.008) and crude tissue homogenates (131 ± 53 vs. 168 ± 38 fmol/mg protein; p < 0.05) from subjects with schizophrenia compared to that in controls. By contrast, there was no significant difference in radioligand binding to the membrane-enriched (155 ± 95 vs. 145 ± 48 fmol/mg protein; p = 0.72) or cytosol-enriched (8.6 ± 14 vs. 7.5 ± 10 mol/mg protein; p = 0.85) tissue fraction. Significantly, adding 10â 5 M risperidone or chlorpromazine, as surrogates for residual antipsychotic drugs in the CNS, to crude homogenate from control subjects did not alter [3H]ketanserin binding. Our data therefore is consistent with the hypothesis that apparent decreases in serotonin2A receptors in schizophrenia are due to altered levels of a regulatory factor(s) that modulates the binding of ligands to the serotonin2A receptor and that separating the membrane and cytosol removes this regulatory control.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Schizophrenia Research - Volume 104, Issues 1â3, September 2008, Pages 185-197
Journal: Schizophrenia Research - Volume 104, Issues 1â3, September 2008, Pages 185-197
نویسندگان
Brian Dean, Nicola Crossland, Simone Boer, Elizabeth Scarr,