Microbial transformation of 20(R)-panaxadiol by Absidia corymbifera AS 3.3387

• Biotransformation of 20(R)-panaxadiol has been investigated.
• Total seven biotransformation products including six new ones were identified.
• Their cytotoxicity activities for four cancer cell lines were assayed.

The microbial transformation of 20(R)-panaxadiol (1) by Absidia corymbifera AS 3.3387 was investigated. Seven oxidized and hydroxylated products 20(R),25-epoxy-12β-hydroxydammaran- 3-one (2), 20(R),25-epoxy-7β,12β,24α-trihydroxydammaran-3-one (3), 20(R),25-epoxy- 7β,12β,24β-trihydroxydammaran-3-one (4), 20(R),25-epoxy-12β,15α,24α-trihydroxydammaran- 3-one (5), 20(R),25-epoxy-12β,15α,24β-trihydroxydammaran-3-one (6), 20(R),25-epoxy- 7β,12β,24β-trihydroxydammar-15-en-3-one (7), and 20(R),25-epoxy-7β,12β,24β-trihydroxy- 15,30-cyclodammaran-3-one (8) were obtained. Six metabolites 3–8 are new compounds and are being reported here for the first time. Structures of the new metabolites were elucidated by 1-D (1H, 13C), 2-D NMR (HSQC, HMBC, and ROESY) and HR-MS analyses. In addition, the cytotoxicity of substrate and all transformed products was evaluated by MTT assay using a panel of four human tumor cell lines (Du-145, Hela, HepG2, and MCF-7 cells) and one normal cell line Vero.

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