Immobilization of aminoacylase on electrospun nanofibrous membrane for the resolution of dl-theanine

• Aminoacylase was immobilized on polyvinyl alcohol-based nanofibrous membranes.
• The immobilized aminoacylase showed the optimal catalytic conditions at pH 8 and 52 °C.
• Thermostability and storage stability of the immobilized aminoacylase were significantly improved.
• The nanofibrous-immobilized aminoacylase could be used to produce pure l-theanine.

The enzyme aminoacylase was used to produce l-theanine from its derivative N-acetyl-dl-theanine. For stabilization purposes the aminoacylase was immobilized on polyvinyl alcohol-based nanofibrous membranes generated by electrospinning. The immobilized aminoacylase exhibited better resistance to changes in temperature and pH than the free enzyme with optimal conditions being pH 8 and 52 °C. Under these conditions, Km values were two to three times higher than those of the free enzyme (3.6 mM). Thermostability was also significantly improved; the activity of the immobilized enzyme was retained at approximately 70% after 6 days at 52 °C. These results indicate potential applications of nanofibrous-immobilized aminoacylase for industrial production of pure l-theanine.

Figure optionsDownload as PowerPoint slide