کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
69496 48772 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production and characterization of lipases and immobilization of whole cell of the thermophilic Thermomucor indicae seudaticae N31 for transesterification reaction
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Production and characterization of lipases and immobilization of whole cell of the thermophilic Thermomucor indicae seudaticae N31 for transesterification reaction
چکیده انگلیسی


• The first report of a lipase by the thermophilic fungus Thermomucor indicae seudaticae.
• The advantage of whole hyphae immobilization as a simple and cheap process for biocatalysts production.
• Two different extracellular lipases produced by distinct fermentation processes (SSF and SmF).

Among 54 thermophilic fungi tested for lipase production, the highest yield was obtained for Thermomucor indicae seudaticae in submerged (SmF) and solid state fermentation (SSF), being that species selected for studies of fermentative parameters. Some modifications of the culture media afforded an improvement of 15 fold in the lipase activity in SSF and 8 fold in SmF. Furthermore, the lipases produced through both SSF and SmF exhibited maximum lipolytic activity at 40 °C but these lipases were different since the enzyme from SmF presented maximum activity at neutral pH (6.0–7.0) whereas the lipase from SSF displayed maximum activity at acidic pH (3.0–4.0). The fungus was grown on loofah sponges, and the immobilized hyphae showed 108 U g− lipolytic activity, a profile including both alkaline and acidic activities. The esterification and transesterification yields were 28% and 13.2% using oleic acid and soybean oil, respectively. Those are essential reactions for biodiesel production. Thus, the lipase production of both the immobilized whole cells as well as the cultivated fungus T. indicae seudaticae N31 may be considered promising in synthesis reactions.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 107, September 2014, Pages 106–113
نویسندگان
, , , , , , , , ,