Immobilization of horseradish peroxidase (HRP) on polyimide nanofibers blending with carbon nanotubes

• Polyimide/MWCNTS nanofibers are electrospun for enzyme immobilization.
• The retention activity of immobilized HRP is obviously improved by MWCNTS.
• Electron transfer among “HRP I”, “HRP II” and MWCNTs is facilitated.

Carbon nanotubes are widely applied in electronics, which offers great potential for enhancing the activity of redox enzyme. In this work, polyimide (PI) was blended with multiwalled carbon nanotubes (MWCNTs) and then electrospun into nanofibers for the immobilization of horseradish peroxidase (HRP), a redox enzyme. SEM and TEM were used to characterize the surface morphology of the PI/MWCNTs nanofibers and to analyze the protruding parts induced by the blending of MWCNT. The results indicate that, compared to the virgin PI nanofibers, the blending of MWCNTs increases the retention activity of immobilized HRP from 2.38% to 12.50%. Circular dichroism and UV–vis molecular absorption spectrometry were used to explore the interactions between HRP and MWCNTs. It seems that MWCNTs increase the enzyme activity by the facilitation of electron transfer among the catalytic intermediates “HRP I”, “HRP II” and MWCNTs.

Figure optionsDownload as PowerPoint slide