کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
69573 48781 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of hydroxamic acids by immobilized Pseudomonas aeruginosa cells: Kinetic analysis in reverse micelles
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Production of hydroxamic acids by immobilized Pseudomonas aeruginosa cells: Kinetic analysis in reverse micelles
چکیده انگلیسی


• Free and immobilized cells of Pseudomonas aeruginosa L10 contains biocatalyst amidase for hydroxamate derivatives synthesis.
• Kinetic studies using a broad range of substrates in both media.
• The immobilization in reverse micelles of cationic surfactant increases the biocatalyst affinity for the substrates.
• Reverse micellar medium allowed obtaining higher biocatalyst stability and a greater percentage of substrate conversion.

Intact cells from Pseudomonas aeruginosa strain L10 containing amidase were used as biocatalysts both free and immobilized in a reverse micellar system. The apparent kinetic constants for the transamidation reaction in hydroxamic acids synthesis, were determined using substrates such as aliphatic, amino acid and aromatic amides and esters, in both media. In reverse micelles, Km values decreased 2–7 fold relatively to the free biocatalyst using as substrates acetamide, acrylamide, propionamide and glycinamide ethyl ester. We have concluded that overall the affinity of the biocatalyst to each substrate increases when reactions are performed in the reversed micellar system as opposed to the buffer system. The immobilized biocatalyst in general, exhibits higher stability and faster rates of reactions at lower substrates concentration relatively to the free form, which is advantageous. Additionally, the immobilization revealed to be suitable for obtaining the highest yields of hydroxamic acids derivatives, in some cases higher than 80%.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 93, September 2013, Pages 28–33
نویسندگان
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