کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
69716 | 48789 | 2014 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development of a mixed solvent system for the efficient resolution of (R, S)-2-octanol catalyzed by magnetite-immobilized lipase Development of a mixed solvent system for the efficient resolution of (R, S)-2-octanol catalyzed by magnetite-immobilized lipase](/preview/png/69716.png)
• Solvent structure affects resolution performance of (R, S)-2-octanol by lipase.
• Acyclic linear solvent resulted in efficient enantioselectivity and enzymatic activity.
• Mixture of acetone and carbon tetrachloride was developed for enhancing enzymatic resolution.
In order to find a suitable reaction system for the enzymatic resolution of (R, S)-2-octanol, the effects of the molecular structure of the solvent on the enantioselectivity (E) and enzymatic activity of Yarrowia lipolytica lipase (YLL) immobilized onto magnetic nanoparticles were systematically analyzed. Both the E and enzymatic activity of the reaction in an acyclic, structurally linear solvent were higher than those in the corresponding branched chain solvent or cyclic solvent. In a mixed solvent system with acetone and carbon tetrachloride (v/v = 3:7), the immobilized YLL exhibited high enantioselectivity, activity, and reusability. The thermodynamic analysis showed that the enantiomer discrimination was enthalpy-driven at all temperatures tested. These results present new opportunities and challenges for understanding and intensifying the enzymatic resolution process of (R, S)-2-octanol by designing suitable solvent system.
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Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 101, March 2014, Pages 23–27