Improvement of the thermal stability and aldehyde tolerance of deoxyriboaldolase via immobilization on nano-magnet material

• The deoxyriboaldolase was efficiently immobilized on a nano-magnet material.
• The immobilized EcoDERA shows a better tolerance of acetaldehyde.
• The immobilized EcoDERA shows higher thermal stability.
• The immobilized EcoDERA can be easily separated from the reaction system by using only a magnetic-iron.

EcoDERA2 as efficiently immobilized on GA-APTES-NSM1 with a yield of over 75% After incubation of the free EcoDERA in 300 mM acetaldehyde for 3 h, no activity was observed, while in contrast, the EcoDERA-GA-APTES-NSM2 retained more than 60% of its activity after a 3 h incubation. The EcoDERA-GA-APTES-NSM possessed 65.7% of its initial activity after incubation at 80 °C for 10 min, whereas, the free enzyme lost almost all of its activity under these conditions. The immobilized enzyme can be easily separated from the reaction system by using only a magnetic-iron.These results suggest the potential utility of GA-APTES-NSM as an immobilization support for EcoDERA.

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